Genomic DNA Extraction Kit (Spin Column)

Overview

At a Glance

• Column-based genomic DNA purification kit built on a silica-membrane spin column plus a complete buffered system
• After protease K lysis, DNA binds specifically to the membrane under high-salt, tuned-pH conditions while proteins and contaminants pass through
• Two wash steps remove impurities; DNA is eluted under low-salt, high-pH conditions in 50-200 uL of TE buffer
• Extracted DNA is high-molecular-weight, high-purity, and batch-to-batch consistent — directly usable in downstream molecular workflows
• Typical protocol completes in about 1 hour (tissue digestion may take longer)

Key Features

• Broad sample compatibility: whole blood (~200 uL), cultured cells (10^6-10^7), and animal tissue (~30 mg) from a single kit format
• Consistent yields: 3-10 ug from blood, 5-30 ug from cells, 10-30 ug from tissue (manufacturer data; varies with sample type and handling)
• Genuinely downstream-ready: eluted DNA goes straight into PCR, restriction digestion, library construction, and Southern hybridization without further cleanup
• Complete system in one box: lysis, binding, deproteinization, wash, and elution buffers plus Proteinase K, spin columns, and 2 mL collection tubes
• Room-temperature shipping and storage: 15-30 C dry storage, ~15-month shelf life — no cold chain required

Specifications (manufacturer data)

Protocol Overview

• Lyse: suspend the sample in lysis buffer, add Proteinase K, mix; digest tissue at 56 C until fully dissolved
• Bind: add binding buffer, incubate at 70 C for ~10 min until the solution clears; add absolute ethanol, load onto the spin column, centrifuge 30 s at 12,000 rpm, discard flow-through
• Wash: sequential centrifugation with deproteinization buffer, then two washes
• Dry: spin the empty column, then leave the lid open for several minutes - residual ethanol inhibits downstream PCR and restriction digestion
• Elute: apply 50-200 uL TE buffer to the membrane center, stand 2-5 min at room temperature, centrifuge; a second elution improves recovery
• Store purified DNA at -20 C to prevent degradation

Handling Notes

• Add ethanol before first use: the wash and deproteinization buffers ship as concentrates - add absolute ethanol per the volume printed on each bottle before starting
• Do not skip the dry spin: carryover ethanol is the most common cause of failed downstream enzymatic reactions
• Respect the minimum elution volume: eluting below 50 uL harms recovery
• Store purified DNA at -20 C for long-term use; avoid repeated freeze-thaw cycles

Applications

• Genomic DNA extraction from whole blood, cultured cells, and animal tissue samples
• Template preparation for PCR, qPCR, and genotyping workflows
• Input material for restriction digestion, library construction, and Southern blotting
• Paired sample prep with protein extraction - see our RIPA Lysis Buffer for the total-protein track from the same samples

Research Use Only

For Research Use Only. Not for use in clinical diagnosis or therapeutic procedures. Packaging and documentation do not reference medical or diagnostic use; shipped via the research-reagent export channel.

Ordering & Documentation

• Pack sizes: 50 preps / 200 preps; optional RNase A and red blood cell lysis buffer add-ons
• Certificate of Analysis, quality documentation, and lead times are confirmed per inquiry - Send us an inquiry and we will respond with verified details.