RNA-LNP Encapsulation Efficiency Assay Kit (RiboGreen-based)
Plate-based fluorescence assay for RNA encapsulation efficiency in LNP formulations — total vs. free RNA in 30–60 minutes on any standard fluorescence microplate reader.
Ships with certificate of analysis
For Research Use OnlyOverview
How it works
The assay uses a fluorescent RNA-binding dye that is virtually non-fluorescent in solution and fluoresces strongly upon binding RNA (Ex 490 nm / Em 525 nm). Each sample is split into two aliquots: one is measured directly to quantify free (unencapsulated) RNA, the other is treated with the supplied lysis buffer to release total RNA. Encapsulation efficiency is calculated from the difference: EE% = (total − free) / total × 100.
The kit is optimized for the 96-well plate format and runs on any standard fluorescence microplate reader — no HPLC, no capital equipment, results in 30–60 minutes.
Performance
| Linear range (high dye) | 2.5–50 ng/mL |
|---|---|
| Linear range (low dye) | 10 ng/mL–1 µg/mL |
| Combined linear range | 2.5 ng/mL–1 µg/mL |
| Assay format | 96-well plate, 200 µL per well |
| Runs per kit | 200 (high dye) · 2,000 (low dye) |
| Excitation / emission | 490 nm / 525 nm |
| Incubation | 2–5 min, protected from light |
| Total workflow | 30–60 minutes |
| Reference criterion | EE% ≥ 85–90% for well-formed LNPs |
Kit contents
| RiboGreen reagent | 100 µL (amber tube) |
|---|---|
| 20× TE concentrate (RNase-free) | 25 mL |
| RNA quantification standard | 2 µg |
| 10× LNP lysis buffer | 25 mL |
| Handbook | 1 copy |
Storage & shipping
| Storage | −20 °C, protected from light |
|---|---|
| Shelf life | 6 months from manufacture |
| Shipping | Cold-chain shipment |
Interference & sample preparation
The assay tolerates nucleotides, salts, urea, ethanol, chloroform, detergents, proteins and agarose. DNA interferes with quantification — treat samples with DNase if DNA is present. For complex matrices we recommend dialysis (MWCO 20 kDa, ≥ 2 h) followed by 0.22 µm filtration. Use RNase-free plasticware throughout and avoid glass containers.
Required but not supplied: samples, 1× PBS and a fluorescence reader.
| Product | RNA-LNP Encapsulation Efficiency Assay Kit (RiboGreen-based) |
|---|---|
| Category | QC & Analysis |
| Documentation | Certificate of analysis provided with every lot |
| Intended use | For Research Use Only (RUO). Not for diagnostic or therapeutic use. |
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