RIPA Lysis Buffer — Total Protein Extraction Reagent

Overview

At a Glance

Ready-to-use RIPA lysis buffer for rapid total protein extraction from cultured cells and tissues. A balanced mixed-detergent system — 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS — disrupts cells on contact, while built-in protease and phosphatase inhibitors (sodium orthovanadate, sodium fluoride, EDTA, leupeptin and others) preserve target proteins and phosphorylation modifications. Spin, collect the supernatant, and go straight to Western blot, IP / co-IP, PAGE or ELISA.

Key Features

• Seconds-fast lysis: cells disrupt within 1–2 seconds of contact — no sonication needed
• Protein-protecting formulation: the built-in protease + phosphatase inhibitor system preserves targets and phosphorylation states during lysis
• Three strength options plus a Complete format: match lysis stringency to your sample; the Complete format adds a de-viscosity solubilizer that eliminates stringy lysate and raises typical protein yield by ~5–25%
• Broad sample compatibility: animal and plant cells, tissues, fungi and bacteria
• High-throughput friendly: the whole workflow is pipette-and-spin — no specialized equipment

Strength Options

Pack Sizes

A Complete format (standard buffer plus de-viscosity solubilizer — no flocculent sticky lysate, ~5–25% higher yield) is available on request.

Specifications

Protocol (Short)

• 1. Prepare: thaw and mix; add PMSF (1 mM final) or an inhibitor cocktail immediately before use
• 2. Lyse: adherent cells — aspirate medium, rinse once with PBS, add 150–250 µL per well and pipette briefly (lysis completes within seconds of contact). Tissue — mince, add 150–250 µL per 20 mg, homogenize or grind
• 3. Incubate on ice. With the Complete format, sticky viscosity typically disappears within 1–2 minutes
• 4. Clarify: spin at 10,000–14,000 x g for 3–5 min, take the supernatant and proceed to Western blot, IP / co-IP, PAGE or ELISA

Applications

• Total protein extraction from cells and tissues ahead of Western blot and PAGE
• Sample preparation for immunoprecipitation (IP), co-IP and ELISA
• Downstream expression validation after transfection or LNP delivery — pairs naturally with our Universal Lipid-Based Transfection Reagent and the CCK-8 Viability Assay: transfect, check viability, lyse, blot
• Lysis of animal, plant, fungal and bacterial samples
• Phosphorylated-protein and subcellular-fraction studies (select inhibitor variants per inquiry)

Handling Notes

• PMSF is not included and must be added fresh. PMSF degrades rapidly in aqueous solution — add immediately before lysis; never store pre-mixed.
• Do not quantify RIPA lysates with the Bradford method — the detergents interfere. Use a BCA assay instead.
• Aliquot on first thaw and avoid repeated freeze-thaw cycles; keep the entire workflow on ice or at 4 C.

Research Use Only

For Research Use Only. Not for clinical or diagnostic use.

Ordering & Documentation

Available strengths, pack sizes, the Complete format, inhibitor variants, COA, MSDS, ISO documentation, FOB pricing, MOQ and lead time are confirmed per inquiry: Send us an inquiry