RNA Quantitation Kit (SkyGreen-based, 96-well)
Fluorescence RNA quantitation with standard curve in every run — same dye platform as our EE assay, for purified or free RNA samples.
Catalog no. 230650-3 · Ships with certificate of analysis
For Research Use OnlyOverview
Overview
The kit quantifies RNA in solution using the SkyGreen fluorescent dye: virtually non-fluorescent until bound to RNA, with signal linear to RNA concentration. An RNA standard is included for building the standard curve in the same plate, so every run is calibrated on the spot.
Runs on any fluorescence microplate reader, standard fluorometer or filter fluorimeter — the same instrument you would use for the encapsulation efficiency workflow.
Performance
| Linear range (high dye) | 2.5–50 ng/mL |
|---|---|
| Linear range (low dye) | 10 ng/mL–1 µg/mL |
| Combined linear range | 2.5 ng/mL–1 µg/mL |
| Assay format | 96-well plate |
| Size | 200 tests |
| RNA standard | included, for standard curve |
| Reader | fluorescence microplate reader / fluorometer |
| Storage | −20 °C, cold-chain shipping |
| Lead time | 3–5 days |
Kit contents
| SkyGreen fluorescent dye | 1 vial |
|---|---|
| 20× TE concentrate | 1 bottle |
| RNA quantitation standard | 1 vial |
| Handbook | 1 copy |
Note: this kit does not contain LNP lysis buffer and is not intended for demulsification of lipid particles — for encapsulated samples use the RNA-LNP Encapsulation Efficiency Assay Kit. It is the companion tool when you need absolute RNA concentration of purified samples, in-process controls or free-RNA fractions.
Interference
The assay tolerates nucleotides, salts, urea, ethanol, chloroform, detergents, proteins and agarose. DNA interferes with quantification — treat samples with DNase if DNA is present.
| Product | RNA Quantitation Kit (SkyGreen-based, 96-well) |
|---|---|
| Catalog no. | 230650-3 |
| Category | QC & Analysis |
| Documentation | Certificate of analysis provided with every lot |
| Intended use | For Research Use Only (RUO). Not for diagnostic or therapeutic use. |
Related products
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Get a quote for RNA Quantitation Kit (SkyGreen-based, 96-well)
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