Nanoparticle Size & Zeta Potential Analyzer (DLS)

Overview

At a Glance

• Integrated light-scattering platform combining DLS (size), ELS/PALS (zeta potential), SLS (molecular weight), and transmittance detection in one benchtop instrument
• Size by Brownian motion: scattering-intensity fluctuations are autocorrelated to obtain the diffusion coefficient, then converted to hydrodynamic diameter via the Stokes-Einstein equation
• Zeta by phase-analysis electrophoretic light scattering (PALS): electrophoretic mobility of charged particles in an applied field is converted to zeta potential
• One run delivers the core LNP QC triad: hydrodynamic size, PDI, and zeta potential - the two release-critical attributes tracked alongside encapsulation efficiency

Key Features

• Wide size range: 0.3 nm to 15 um hydrodynamic diameter, covering LNP, liposome, exosome, protein, and polymer systems
• Multi-angle detection (173 deg backscatter + 90 deg + 11.2 deg): high-concentration samples measured directly by backscatter; forward angles cover low-concentration and large-particle work
• Tiny sample requirement: minimum ~3 uL with the low-volume cuvette; standard measurements ~1 mL
• Concentration tolerance up to 40% w/v - dilute-averse formulations can often be measured as-is
• Trend analysis: size / zeta tracked vs. temperature, time, or pH, with transition points such as Tm reported automatically
• Broad temperature control (-15 C to 120 C) for stability studies and thermal scans
• Flexible algorithms: Cumulants, CONTIN, and NNLS for size distribution analysis

Specifications (manufacturer data)

Sample Handling Notes

• Dust and aggregates destroy DLS data: samples must be well dispersed, free of bubbles, and free of large particles - filter or centrifuge to remove dust where appropriate
• Use the low-volume cuvette for precious samples; use the 173 deg backscatter angle for high-concentration or turbid samples
• Zeta measurements require the dedicated electrode cell: watch medium conductivity and follow the cell's rated conductivity range
• Trend runs (temperature / pH titration) are the fastest way to assess formulation stability and colloidal robustness

Applications

• LNP and liposome QC: size, PDI, and zeta potential release testing - the core physical attributes for LNP formulation programs
• Formulation screening and comparability studies across lipid compositions and process changes
• Stability and stress studies: size / zeta vs. temperature, pH, and time, with Tm detection
• Colloidal stability judgment ahead of storage, shipping, and administration studies
• General nanoparticle characterization: exosomes, proteins, polymers, emulsions in chemistry, biotech, pharma, food, and materials labs

Research Use Only

For Research Use Only. The instrument and its documentation do not reference medical or diagnostic use.

Ordering & Documentation

• Configurations, optional accessories (low-volume cuvettes, electrode cells, titrator), and pricing are confirmed per inquiry
• Specifications, documentation, and lead times are verified per order - Send us an inquiry and we will respond with a detailed quotation.