E. coli Residual RNA Detection Kit (probe-based qRT-PCR)
Probe-based qRT-PCR kit for residual E. coli RNA — internal control against false negatives, sensitivity to 0.001 pg/µL, five-log linear range.
Catalog no. 17-39800-resiR · Ships with certificate of analysis
For Research Use OnlyOverview
Overview
A probe-based qRT-PCR kit for detecting and quantifying residual Escherichia coli RNA in biological samples and process intermediates. Primers and probes target E. coli conserved regions with no cross-reactivity against RNA of other organisms, making it a specific tool for host-cell RNA impurity testing.
Each run includes an internal amplification control (to exclude false negatives caused by inhibition) and a positive control for building the quantitative standard curve.
Performance
| Sensitivity | 0.001 pg/µL |
|---|---|
| Quantitative linear range | at least 5 orders of magnitude |
| Result type | quantitative and qualitative |
| Internal control | included — excludes false negatives |
| Positive control | included — for standard curve |
| Specificity | E. coli conserved regions; no cross-reaction with other organisms’ RNA |
Specifications
| Size | 50 tests, 20 µL reaction system |
|---|---|
| Storage | −20 °C, cold-chain shipping |
| Lead time | 2–3 weeks |
| Intended use | Research Use Only |
Applications
Residual host-cell RNA QC for recombinant products expressed in E. coli; bioprocess impurity monitoring; contamination investigation in RNA workflows.
| Product | E. coli Residual RNA Detection Kit (probe-based qRT-PCR) |
|---|---|
| Catalog no. | 17-39800-resiR |
| Category | QC & Analysis |
| Documentation | Certificate of analysis provided with every lot |
| Intended use | For Research Use Only (RUO). Not for diagnostic or therapeutic use. |
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